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Image Search Results
Journal: ACS Catalysis
Article Title: Ultrahigh-Throughput Activity Engineering of Promiscuous Amidases through a Fluorescence-Activated Cell Sorting Assay
doi: 10.1021/acscatal.5c01903
Figure Lengend Snippet: FACS-based detection of amidase activity. ( A ) Overview of the FACS-based assay principle. Fluorescence histograms of E. coli BL21(DE3)-Gold cells expressing GST only as a negative control ( B ) or co-expressing GST and the amidase Pd Amd ( C ) or Sa Amd ( D ), respectively. Amidase activity in whole cells towards 1 was measured by flow cytometry (FL3-UV: λ ex = 375 nm/λ em = 440 nm); 1 and 1-GSH do not exhibit fluorescence.
Article Snippet:
Techniques: Activity Assay, Fluorescence, Expressing, Negative Control, Flow Cytometry